Phone +49 (0)251-53406-505/858
Mobile phone +49 (0)173-7250-297
Fax +49 (0)251-53406-857
E-Mail dna@chembiotech.de
© ChemBiotech 2023
Mobile phone +49 (0)173-7250-297
Fax +49 (0)251-53406-857
E-Mail dna@chembiotech.de
© ChemBiotech 2023
N3-Cyanoethyl-dT CE-phosphoramidite
Modified Phosphoramidites , class Other modifications
| Catalog # | CBT-P-110 |
| Compound | N3-Cyanoethyl-dT CE-phosphoramidite |
| Chemical Name | N3-(2-Cyanoethyl)-1-[2-deoxy-5-O-(4,4'-dimethoxytriphenylmethyl)-ß-D-erythro-pentofuranosyl]-thymidine 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite |
| Formula | C43H52N5O8P |
| Molecular Weight | 797.88 g/mol |
| Properties | Storage at -18°C The cyanoethyl group is commonly used as P(III) protecting group and is cleaved during oligonucleotide synthesis by beta-elimination under alkaline conditions affording acrylonitrile. Acrylonitrile acts as alkylating agent and partially adds to N3 of dT. As a result, a full length oligonucleotide is obtained in which thymine is partially replaced by an N3-cyanoethylthymine residue. We offer the phosphoramidite building block CBT-P-110 as a standard to elucidate side product formation during oligonucleotide synthesis. |
| Appearance | Colorless foam |
| References | D. C. Capaldi, H. Gaus, A. H. Krotz, J. Arnold, R. L. Carty, M. N. Moore, A. N. Scozzari, K. Lowery, D. L. Cole, V. T. Ravikumar, Org. Process Res. Dev. 2003, 7, 832. |
| Quantity | Pricing (EUR) |
| 100 µmol | |
| 250 mg | |
| 1.0 g |
For bulk quantities request by e-Mail: dna@chembiotech.de or phone: +49 251 53406 858 or +49 251 53406 505.