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N3-Cyanoethyl-dT CE-phosphoramidite
Modified Phosphoramidites , class Other modifications
N<sup>3</sup>-Cyanoethyl-dT CE-phosphoramidite
Catalog # CBT-P-110
Compound N3-Cyanoethyl-dT CE-phosphoramidite
Chemical Name N3-(2-Cyanoethyl)-1-[2-deoxy-5-O-(4,4'-dimethoxytriphenylmethyl)-ß-D-erythro-pentofuranosyl]-thymidine 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula C43H52N5O8P
Molecular Weight 797.88 g/mol
Properties Storage at -18°C

The cyanoethyl group is commonly used as P(III) protecting group and is cleaved during oligonucleotide synthesis by beta-elimination under alkaline conditions affording acrylonitrile. Acrylonitrile acts as alkylating agent and partially adds to N3 of dT. As a result, a full length oligonucleotide is obtained in which thymine is partially replaced by an N3-cyanoethylthymine residue. We offer the phosphoramidite building block CBT-P-110 as a standard to elucidate side product formation during oligonucleotide synthesis.
Appearance Colorless foam
References D. C. Capaldi, H. Gaus, A. H. Krotz, J. Arnold, R. L. Carty, M. N. Moore, A. N. Scozzari, K. Lowery, D. L. Cole, V. T. Ravikumar, Org. Process Res. Dev. 2003, 7, 832.
QuantityPricing (EUR)
100 µmol 160,00
250 mg 290,00
1.0 g 950,00
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