|Compound||7-Bromo-2-amino-8-aza-7-deaza-dA (formyl, di-n-butyl) CE-phosphoramidite|
|Chemical Name||3-Bromo-1-[2-deoxy-5-O-(4,4’-dimethoxytrityl)-ß-D-erythro-pentofuranosyl]-4-[[(di-n-butylamino)methylidene]amino]-6-[(formyl)amino)]-1H-pyrazolo[3,4-d]pyrimidin-4,6-diamine 3’-[(2-cyanoethyl)-N,N-(diisopropyl)]-phosphoramidite|
|Molecular Weight||1014.99 g/mol|
|Properties||Storage at -18°C
Our phosphoramidite building block CBT-P-102 can be employed in solid-phase oligonucleotide synthesis under standard conditions. Also, standard protocols can be used for the deprotection of the resulting oligonucleotides. 7-Bromo-2-amino-8-aza-7-deaza-dA has a significantly stabilized N-glycosylic bond compared to the corresponding, rather labile 2,6-diaminopurine 2'-deoxyribonucleoside analog and forms a tridentate base pair with dT, which is as strong as the canonical dG-dC pair. In addition, as a constituent of oligonucleotides, this nucleoside shows similiar base pair discriminatory properties as dA.
|References||F. Seela, G. Becher, Nucleic Acids Res. 2001, 29, 2069.
G. Becher, F. Seela, J. He, Helv. Chim. Acta 2001, 84, 1048.