|Compound||7-Deaza-dG (form) CE-phosphoramidite|
|Chemical Name||7-[2-Deoxy-5-O-(4,4'-dimethoxytrityl)-ß-D-erythro-pentofuranosyl]-3,7-dihydro-2-[((dimethylamino)methylidene)amino]-4H-pyrrolo[2,3-d]pyrimidin-4-one 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite|
|Molecular Weight||823.92 g/mol|
|Properties||Storage at -18°C
Phosphoramidites of 7-deaza-2’-deoxyguanosine (7-deaza-dG) were first prepared in 1985 by Seela and co-workers. Oligonucleotides containing 7-deaza-dG give higher sensitivity in MALDI-TOF spectrometry due to the rather stable glycosidic bond under acidic conditions. The incorporation of this modified nucleoside in place of dG destroys dG-quartets, thereby prohibiting oligonucleotide aggregation during gel electrophoresis or PCR-amplification. Multiple incorporations of 7-deaza-dG might require milder oxidation reagents or the use of our product CBT-P-160.
|References||F. Seela, H. Driller, Nucleic Acids Res. 1985, 13, 911.
F. Seela, H. Driller, Nucleic Acids Res. 1989, 17, 901.