|Compound||7-Deaza-dA (ibu) CE-phosphoramidite|
|Chemical Name||7-[2-Deoxy-5-O-(4,4'-dimethoxytriphenylmethyl)-ß-D-erythro-pentofuranosyl]-4-[(isobutyryl)amino]-7H-pyrrolo[2,3-d]pyrimidine 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite|
|Molecular Weight||822.94 g/mol|
|Properties||Storage at -18°C
Phosphoramidites of 7-deaza-2’-deoxyadenosine (7-deaza-dA) were first described by Seela and coworkers. As the nucleoside is remarkably stable under acidic conditions, oligonucleotides do not “depurinate”. Thus, they are used in MALDI-TOF spectrometry to enhance peak sensitivity. The nucleoside also prevents cleavage of oligonucleotides by nucleases. In addition, the absence of nitrogen-7 provides useful information in the detection of DNA-enzyme binding sites.
|References||F. Seela, A. Kehne, Biochemistry 1985, 24, 7556.
F. Seela, A. Kehne, Biochemistry 1987, 26, 2232.